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A Pan-Amazonian species delimitation: high species diversity within the genus Amazophrynella (Anura: Bufonidae) ArchiMer
Rojas, Rommel R.; Fouquet, Antoine; Ron, Santiago R.; Hernandez-ruz, Emil Jose; Melo-sampaio, Paulo R.; Chaparro, Juan C.; Vogt, Richard C.; De Carvalho, Vinicius Tadeu; Pinheiro, Leandra Cardoso; Avila, Robson W.; Farias, Izeni Pires; Gordo, Marcelo; Hrbek, Tomas.
Amphibians are probably the most vulnerable group to climate change and climate-change associate diseases. This ongoing biodiversity crisis makes it thus imperative to improve the taxonomy of anurans in biodiverse but understudied areas such as Amazonia. In this study, we applied robust integrative taxonomic methods combining genetic (mitochondrial 16S, 12S and COI genes), morphological and environmental data to delimit species of the genus Amazophrynella (Anura: Bufonidae) sampled from throughout their pan-Amazonian distribution. Our study confirms the hypothesis that the species diversity of the genus is grossly underestimated. Our analyses suggest the existence of eighteen linages of which seven are nominal species, three Deep Conspecific Lineages, one...
Tipo: Text Palavras-chave: Integrative taxonomy; Cryptic diversity; Species delimitation; Amphibians; Amazon.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00660/77219/78676.pdf
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Biodiversity inventory of the grey mullets (Actinopterygii: Mugilidae) of the Indo‐Australian Archipelago through the iterative use of DNA‐based species delimitation and specimen assignment methods ArchiMer
Delrieu‐trottin, Erwan; Durand, Jean‐dominique; Limmon, Gino; Sukmono, Tedjo; Kadarusman,; Sugeha, Hagi Yulia; Chen, Wei‐jen; Busson, Frédéric; Borsa, Philippe; Dahruddin, Hadi; Sauri, Sopian; Fitriana, Yuli; Zein, Mochamad Syamsul Arifin; Hocdé, Régis; Pouyaud, Laurent; Keith, Philippe; Wowor, Daisy; Steinke, Dirk; Hanner, Robert; Hubert, Nicolas.
DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications...
Tipo: Text Palavras-chave: Coral Triangle; Cryptic diversity; DNA barcoding; Reference library; Taxonomic gap.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71810/70300.pdf
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DNA barcodes for soil animal taxonomy PAB
Rougerie,Rodolphe; Decaëns,Thibaud; Deharveng,Louis; Porco,David; James,Sam W.; Chang,Chih-Han; Richard,Benoit; Potapov,Mikhail; Suhardjono,Yayuk; Hebert,Paul D.N..
The biodiversity of soil communities remains very poorly known and understood. Soil biological sciences are strongly affected by the taxonomic crisis, and most groups of animals in that biota suffer from a strong taxonomic impediment. The objective of this work was to investigate how DNA barcoding - a novel method using a microgenomic tag for species identification and discrimination - permits better evaluation of the taxonomy of soil biota. A total of 1,152 barcode sequences were analyzed for two major groups of animals, collembolans and earthworms, which presented broad taxonomic and geographic sampling. Besides strongly reflecting the taxonomic impediment for both groups, with a large number of species-level divergent lineages remaining unnamed so far,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collembola; Cryptic diversity; DNA barcoding; Oligochaeta; Species identification; Taxonomic crisis; Taxonomic impediment.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000800002
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Methodology for Single-Cell Genetic Analysis of Planktonic Foraminifera for Studies of Protist Diversity and Evolution ArchiMer
Weiner, Agnes K. M.; Morard, Raphael; Weinkauf, Manuel F. G.; Darling, Kate F.; André, Aurore; Quillévéré, Frédéric; Ujiie, Yurika; Douady, Christophe J.; De Vargas, Colomban; Kucera, Michal.
Single-cell genetic analysis is an essential method to investigate the biodiversity and evolutionary ecology of marine protists. In protist groups that do not reproduce under laboratory conditions, this approach provides the only means to directly associate molecular sequences with cell morphology. The resulting unambiguous taxonomic identification of the DNA sequences is a prerequisite for barcoding and analyses of environmental metagenomic data. Extensive single-cell genetic studies have been carried out on planktonic foraminifera over the past 20 years to elucidate their phylogeny, cryptic diversity, biogeography, and the relationship between genetic and morphological variability. In the course of these investigations, it has become evident that genetic...
Tipo: Text Palavras-chave: Foraminifera; Protists; Single-cell genetics; Cryptic diversity; Comparability; Methods; Laboratory protocols; Standardization.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00383/49460/49938.pdf
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Population differentiation or species formation across the Indian and the Pacific Oceans? An example from the brooding marine hydrozoan Macrorhynchia phoenicea ArchiMer
Postaire, Bautisse; Gelin, Pauline; Bruggemann, J. Henrich; Pratlong, Marine; Magalon, Helene.
Assessing population connectivity is necessary to construct effective marine protected areas. This connectivity depends, among other parameters, inherently on species dispersal capacities. Isolation by distance (IBD) is one of the main modes of differentiation in marine species, above all in species presenting low dispersal abilities. This study reports the genetic structuring in the tropical hydrozoan Macrorhynchia phoenicea α (sensu Postaire et al., 2016a), a brooding species, from 30 sampling sites in the Western Indian Ocean and the Tropical Southwestern Pacific, using 15 microsatellite loci. At the local scale, genet dispersal relied on asexual propagation at short distance, which was not found at larger scales. Considering one representative per...
Tipo: Text Palavras-chave: Assignment test; Brooding species; Cryptic diversity; Hydrozoa; Microsatellites.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00399/51011/51795.pdf
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